Background:
@EN The various manifestations of intrinsic cutaneous aging may reflect the age-related changes of dermal ground substanecs, the important components of the dermis.
@ES This study was unddetaken to observe whether there is any age-related changes, quantitatively and qualitatively, in dermal glycosaminoglycan(GAG)
@ES Methods:
@EN The Senescence-Accelerated-Mice(SAM) strain "Prone" to develop senescence(P/8), aged 4 days(P-Y a group, n=5) and 4 months old (P-O group, n=5) were used, with SAM strain "Resistance" to senescece(R/1), aged 4 months old (R=O group, n=5). The
whole
skin of SAM was incubated in 0.1 sodum phosphate buffer(NaPB), 2M guanidine-HCI, and 4M guanidine-HCI, sequencially, for the extraction of GAG. The amount of GASwas measured by using alcian lue and by methylene blue metachromatic assay. Uronic
acid(UA)
was estimated emloying carbazole reaction. Extracted skin protein profiles were analyed by SDS-PAGE.
@ES Results:
@EN 1. The total contents of GAG per wet weight of skin, as easured using alcian blue, was highter in R-O group than R-Y and P-Y group, which is statistically significant.
2. The methylene blue metachromatic assay yielded highter absorbance values in 2M guanidine-HCI extract than NaPB extracts.
3. The total contents of UA decreased with aging in R strains, but striking increase was noted in the P strain.
4. On SDS-PAGE, the protein profiles of NaPB extracts showed a similarity to serum protein. 125 kDa protein band was noted only in guanidine-HCI extracts 37, 40kDa protein bands were appeared in 2M, 4M guanidine-HCI extract of R-O group. But
there
was
no significant difference in both strains.
@ES Conclusion:
@EN These findings suggest that macromolecules, such as GAG, is one of the target molecules of the cutaneous aging process, and these change may be related to the age-related changes of dermal water content. (Kor J Dermatol 1994 ; 32(4) :
631~639)
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